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Sains Malaysiana 53(9)(2024): 3149-3158
http://doi.org/10.17576/jsm-2024-5309-19
Combined Anti-Diabetic and
Wound Healing Effects of Binahong Leaf (Basella rubra L.) and
Snakehead Fish (Channa striata) Extracts
in Alloxan-Induced High-Fat Diet Rats
(Kesan Gabungan Anti-Kencing Manis dan Penyembuhan Luka Ekstrak Daun Binahong (Basella rubra L.) dan Ikan Haruan (Channa atriata) pada Tikus Diet
Tinggi Lemak Teraruh Aloksan)
HERU
SASONGKO1,*, INTAN MUSLIMAH1,
SUKMANIA INDRA ALVIANTI NINGSIH1 & SUTARNO2
1Sebelas Maret University, Diploma 3 of Pharmacy,
Vocational School, Jl. Ir Sutami No.36A Surakarta, Central Java Indonesia, 57126
2Sebelas Maret University, Department of Biology,
Faculty of Mathematics and Natural Science, Jl. Ir Sutami No.36A
Surakarta, Central Java Indonesia, 57126
Received: 10 December 2023/Accepted: 30 July 2024
Abstract
Diabetes mellitus is a metabolic disease that
continues to increase in the world. Lifestyle factors cause diabetes cases to
increase uncontrollably. The development of medicines based on natural
ingredients has become a subject of study for researchers throughout the world.
Meanwhile, binahong leaf extract contains flavonoids
and saponins, which can reduce the absorption of glucose. Snakehead fish
extract containing albumin also influences the reduction of diabetic wounds.
The combination of binahong and snakehead fish
extract is expected to synergistically lower blood glucose levels and cure
diabetic wounds. Therefore, this study aimed to determine the anti-diabetic and
wound healing activity resulting from the combination of binahong leaf and snakehead fish extract in rat models of diabetes induced with alloxan
and fat diet. The test animals were divided into six groups with five per
group, namely normal, positive, and negative control, as well as dose 1
(snakehead fish 250 mg/kg and binahong extract 100 mg/kg),
dose 2 (snakehead fish 250 mg/kg and binahong extract
300 mg/kg), and a single dose of snakehead fish extract 500 mg/kg. The
experiment was conducted for 21 days by administering the test solution once a
day. The results showed that administering a combination of binahong extract at a dose of 100 mg/kg and snakehead fish at a dose of 500 mg/kg
significantly reduced blood glucose levels in diabetic rats (p≤0.05).
Treatment using a combination of the two extracts was more effective in
lowering blood sugar levels compared to a single dose of snakehead fish
extract.
Keywords: Albumin;
diabetes mellitus; diabetic wounds; flavonoid
Abstrak
Diabetes mellitus ialah penyakit metabolik yang terus meningkat di seluruh dunia. Faktor gaya hidup menyebabkan kes diabetes meningkat tanpa kawalan. Pembangunan ubat-ubatan berdasarkan bahan-bahan semula jadi telah menjadi subjek kajian bagi penyelidik di seluruh dunia. Sementara itu, ekstrak daun binahong mengandungi flavonoid dan saponin, yang boleh mengurangkan penyerapan glukosa. Ekstrak ikan toman yang mengandungi albumin juga memberi kesan kepada pengurangan luka diabetes. Gabungan ekstrak binahong dan ekstrak ikan toman dijangka secara sinergis mengurangkan tahap glukosa darah dan menyembuhkan luka diabetes. Oleh itu, kajian ini bertujuan untuk menentukan aktiviti anti-diabetes dan penyembuhan luka yang dihasilkan daripada gabungan binahong daun dan ekstrak ikan toman pada model tikus diabetes teraruh diet aloksan dan lemak. Haiwan ujian dibahagikan kepada enam kumpulan dengan lima setiap kumpulan, iaitu normal, positif, dan kawalan negatif, serta dos 1 (ekstrak ikan
toman 250 mg/kg dan ekstrak binahong 100 mg/kg), dos 2 (ekstrak ikan toman 250 mg/kg dan ekstrak binahong 300 mg/kg) dan satu dos ekstrak ikan toman 500 mg/kg. Uji kaji ini dijalankan selama 21 hari dengan pemberian larutan ujian satu kali sehari. Hasilnya menunjukkan bahawa pengambilan gabungan ekstrak binahong pada dos 100 mg/kg dan
ikan toman pada dos 500 mg/kg secara signifikan mengurangkan tahap glukosa darah pada tikus diabetes
(p≤0.05). Rawatan menggunakan gabungan kedua-dua ekstrak adalah lebih berkesan dalam mengurangkan tahap gula darah berbanding dengan satu dos ekstrak ikan toman.
Kata kunci: Albumin; diabetes melitus;
flavonoid; luka diabetes
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*Corresponding author;
email: heru_sasongko@staff.uns.ac.id
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